P-071 L-proline enhances sperm quality and chromatin condensation of normozoospermic men during in vitro incubation

Abstract Study question Does supplementation of human sperm media with L-Proline improve function and chromatin quality during in Vitro Incubation? Summary answer The inclusion L-proline as an antioxidant improves integrity probably by modulating oxidative stress What is known already Infertility affects millions people reproductive age globally. gametes one the most critical aspects that could affect success rate infertility therapy. storage spermatozoa at incubator to achieve capacitation rise DNA fragmentation a result prolonged incubation. Although mild low levels Reactive Oxygen Species (ROS) boost fertilization capacity improving hyperactivation, capacitation, major contributors quality. plays pivotal role many cell metabolism physiology, including osmotic protection design, size, duration study was conducted on thirty-five healthy men attending Motazedi Hospital, Kermanshah Division Andrology, from December 2020 June 2021. Each semen sample equally divided into 4 groups: group control three treatment groups. contained only (Hams, F10 medium supplemented 5 mg/ml HSA). In groups, different concentrations Proline (1, 2, mmol/L) were also added. Participants/materials, setting, methods 35 normozoospermic lifestyles included. Sperm parameters motility, viability, morphology evaluated. ROS, lipid peroxidation (LPO), total (TAC) determined media. analyzed Aniline blue (AB), Toluidine (TB), Chromomycin A3 (CMA3) staining tests. measured dispersion (SCD) test. All assessments after 1, 4, 24 hours Main results chance (2 resulted noticeably improved maintenance motility viability hr incubation compared (P < 0.05). Also, 2 mmol/L able significantly maintain normal other groups 0.01). However, level ROS production non-significantly diminished > terms peroxidation, presence kept MDA lower led TAC level, any Additionally, AB TB tests indicated CMA3 assessment, however, there minor improvement between group. Moreover, reduced fragmented comparison Limitations, reasons for caution Our team unable carry out additional studies verify impact mitochondrial activity, membrane potential, apoptosis validate efficiency safety using such vitro embryonic development live birth rate. Wider implications findings While further are required uncover mechanisms underlying properties Proline, these extend our knowledge male fertility pave way culture procedure routinely used clinics Trial registration number IR.KUMS.REC.1399.625